TABLE 2

Comparison of transcription factor gene expression between Fa2N-4 cells and cryopreserved human hepatocytes

The gene expression levels were evaluated in human hepatocytes and Fa2N-4 cells after the 24- and 48-h adaptation periods, respectively. Data are expressed as means ± S.E.-fold change in human hepatocytes compared with Fa2N-4 cells determined from three independent experiments, as described under Materials and Methods.

Pubmed ID
Gene
mRNA Expression Levels Relative to Fa2N-4
527
455
LHO
DMQ
NM_001621 AhR 0.21 ± 0.09* 0.06 ± 0.02* 0.17 ± 0.06* 0.14 ± 0.04*
NM_002015 FOXO1A 0.27 ± 0.04* 0.02 ± 0.04 0.18 ± 0.02* 0.62 ± 0.64
NM_178849 HNF4α a 0.50 ± 0.07 0.12 ± 0.02* 0.30 ± 0.02* 0.92 ± 0.14
NM_147223 NCOA1 0.56 ± 0.15 0.29 ± 0.13* 0.43 ± 0.08 0.50 ± 0.14
NM_006540 NCOA2 a 0.71 ± 0.04* 0.24 ± 0.07* 0.56 ± 0.14 0.54 ± 0.13
NM_181659 NCOA3 0.40 ± 0.02* 0.17 ± 0.04* 0.42 ± 0.05 0.31 ± 0.08*
NM_006311 NCOR1 a 0.28 ± 0.13* 0.12 ± 0.01* 0.25 ± 0.06* 0.26 ± 0.05*
NM_001077261 NCOR2 0.30 ± 0.08* 0.10 ± 0.01* 0.32 ± 08* 0.32 ± 0.12*
NM_021969 NROB2 a 28.22 ± 2.17* 14.35 ± 6.33* 47.92 ± 2.58* 95.50 ± 13.56*
NM_007121 NR1H2 a 0.32 ± 0.05* 0.12 ± 0.03* 0.35 ± 0.06* 0.35 ± 0.04*
NM_005123 NR1H4 a , b 0.02 ± 0.02 0.01 ± 0.0* 0.06 ± 0.07 0.08 ± 0.10
NM_033013 NR1I2 0.71 ± 0.22 0.32 ± 0.11* 0.65 ± 0.19 2.44 ± 0.56
NM_005122 NR1I3 >100c,* >100c,* >100c,* 93.76 ± 43.56*
NM_001018074 NR3C1 0.36 ± 0.04* 0.06 ± 0.01* 0.31 ± 0.04* 0.19 ± 0.04*
NM_003489 NRIP1 0.27 ± 0.06* 0.19 ± 0.11* 0.31 ± 0.24 0.39 ± 0.26
NM_013261 PPARGC1A a 0.04 ± 0.01* 0.01 ± 0.01 0.06 ± 0.03* 0.06 ± 0.02*
NM_002957 RXRA 0.27 ± 0.05* 0.17 ± 0.05* 0.22 ± 0.02* 0.61 ± 0.15
NM_000545 TCF1 a 0.24 ± 0.08* 0.05 ± 0.02* 0.18 ± 0.03* 0.30 ± 0.01*
NM_001017535
VDR
1.10 ± 0.37
0.14 ± 0.11
0.54 ± 0.13*
0.33 ± 0.15*

  • ↵* P < 0.05, ΔCt value significantly different from that for Fa2N-4 cells.

  • ↵ a Fa2N-4 cells gene expression increased > 10-fold during the 48-h adaptation period.

  • ↵ b Cryopreserved human hepatocyte gene expression increased > 10-fold during the 24-h adaptation period.

  • ↵ c Low gene expression in Fa2N4 cells (Ct >34).