Kinetic parameters for PRO glucuronidation by HLM and recombinant UGT1A9
Parameter | No albumin | BSA | HSA | HSAFAF | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.1% | 1.0% | 2.0% | 0.1% | 1.0% | 2.0% | 0.1% | 1.0% | 2.0% | ||||||||
| HLM | ||||||||||||||||
| Km (μM) | 127 | 19.4 | 15.9 | 15.5 | 155 | 155 | 264 | 15.8 | 10.1 | 10.6 | ||||||
| Vmax (pmol/min · mg) | 967 | 1009 | 1048 | 1048 | 970 | 1013 | 892 | 964 | 926 | 970 | ||||||
| CLint (μl/min/mg) | 8 | 52 | 66 | 68 | 6 | 7 | 3 | 61 | 92 | 92 | ||||||
| UGT1A9 | ||||||||||||||||
| Km (μM) | 41 | 17.6 | 9.1 | 7.2 | 97 | 98 | 99 | 18.1 | 8.8 | 7.2 | ||||||
| Vmax (pmol/min · mg) | 2285 | 2105 | 2213 | 2280 | 2694 | 2352 | 2196 | 2380 | 2150 | 2322 | ||||||
| CLint (μl/min/mg) | 56 | 120 | 243 | 317 | 28 | 24 | 22 | 131 | 244 | 323 | ||||||