TSU-68 hydroxylation by human cDNA-expressed P450 isoforms
TSU-68 (50 μM) was incubated with insect cell microsomes containing cDNA-expressed P450 isoforms in the presence of a NADPH-generating system.
cDNA-Expressed Human P450 isoform | Formation of TSU-68 Metabolite | ||||
|---|---|---|---|---|---|
| M1 | M2 | M3 | |||
| pmol/min/pmol P450 | |||||
| CYP1A1 | 28.8 | 29.1 | 0.57 | ||
| CYP1A2 | 6.5 | 21.1 | 3.9 | ||
| CYP2A6 | —a | — | — | ||
| CYP2B6 | — | — | — | ||
| CYP2C8 | 0.18 | 0.04 | 0.03 | ||
| CYP2C9 | — | — | — | ||
| CYP2C19 | — | — | — | ||
| CYP2D6 | — | 0.21 | — | ||
| CYP2E1 | — | — | — | ||
| CYP3A4 | — | 0.15 | — | ||
↵ a —, not detected.