Enzymatic kinetic parameters for P450 in human liver microsomes
Enzyme | Assay | Type | Km | Vmax |
|---|---|---|---|---|
| μM | pmol/min/mg pro | |||
| CYP1A2 | Phenacetin-O-deethylation | Simple | 75 | 516 |
| CYP2C9 | Diclofenac 4-hydroxylation | Substrate inhibition | 6.8 | 3657 |
| CYP2D6 | Dextromethorphan O-demethylation | Simple | 5 | 1300 |
| CYP3A4 | Testosterone 6β-hydroxylation | Simple | 75 | 6130 |
| CYP3A4 | Midazolam 1-hydroxylation | Substrate inhibition | 3.5 | 1200 |