TABLE 1

Summary of kinetic parameters for carbamazepine 10,11-epoxidation in different in vitro systems

In Vitro System	Type of Kinetics	V_max	K_m or K_s	α	αKs	CL_int or CL_max	CL_int or CL_max
		pmol/min/pmol CYP or mg protein or 10⁶ cells	μM		μM	μl/min/pmol CYP or mg protein or 10⁶ cells	μl/min/g liver
rCYP3A4	Michaelis-Menten	5.3	630	n/a	n/a	0.0083^a	36
rCYP3A5	Sigmoidal^b	10	2300	0.092	210	0.0077	(rCYP3A4 + rCYP3A5)
HLMs	Sigmoidal^b	440	1900	0.012	23	1.1	34
Human hepatocytes	Sigmoidal^b	27	840	0.045	38	0.076	7.5

↵ a The value represents CL_int
↵ b The kinetic parameters were determined using the two-site kinetic model assuming the equivalence of two active sites (Houston and Kenworthy, 2000)