TABLE 1

Nonspecific binding characteristics of saquinavir, ritonavir, and nelfinavir

fu in Incubation Matrix
Loss from Nonspecific Binding
Hepatocyte metabolism buffer (0.2% BSA)
Microsomes (0.2 mg protein/ml)
Hepatocyte uptake buffer (2% BSA)
Microsomes (0.2 mg protein/ml)
Hepatocyte (0.02% BSA, 0.2 × 106 cells/ml)
%
Saquinavir 0.33 0.34 0.15 16 52
Ritonavir 0.5 0.6 0.12 14 39
Nelfinavir
0.52
0.10
0.08
26
44