TABLE 3

Kinetic parameters of saquinavir, ritonavir, and nelfinavir in rat liver microsomes (n = 3) Mean ± S.D. of triplicate in vitro preparations.




Km

Vmax

Clint
μM pmol/min/mg protein ml/min/mg protein
Saquinavir 0.042 ± 0.05 446 ± 89 11 ± 7
Ritonavir 0.0055 ± 0.0041 53.3 ± 16.5 11.9 ± 4.4
Nelfinavir1
0.007 ± 0.002 (0.01)
100 ± 9.7 (120)
15.7 ± 5.4 (11.4)
  • a Values in parentheses denote parameters estimated from a substrate inhibition fit