Sequences of primers and annealing temperatures used for RT-PCR analyses
Target | Primer | Sequence | Positiona | Annealing Temperature |
|---|---|---|---|---|
| °C | ||||
| UGT1A1 | 1A1-S | 5′-CCT TGC CTC AGA ATT CCT TC-3′ | 696-715 | 58 |
| 1A-AS | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 907-929 | ||
| UGT1A3 | 1A3-S | 5′-TGT TGA ACA ATA TGT CTT TGG TCT-3′ | 347-370 | 58 |
| 1A-AS | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 910-932 | ||
| UGT1A4 | 1A4-Sb | 5′-ACG CTG GGC TAC ACT CAA GG-3′ | 277-296 | 58 |
| 1A4-AS | 5′-TCT GAA TTG GTC GTT AGT AAC T-3′ | 587-608 | ||
| UGT1A5 | 1A5-S | 5′-ACA ATA TGT CTT TGA TCA TA-3′ | 353-372 | 58 |
| 1A5-AS | 5′-AGA AAC AGC ATG GCA AAG-3′ | 667-684 | ||
| UGT1A6 | 1A6-S | 5′-AGA GAA TTT CTG CAG GGG TTT T-3′ | 26-47 | 56 |
| 1A6-AS | 5′-TTG GAT TCT TTC AAA AGC-3′ | 195-212 | ||
| UGT1A7 | 1A7-Sc | 5′-TGG CTC GTG CAG GGT GGA CTG-3′ | 2-22 | 58 |
| 1A7-ASc | 5′-TTC GCA ATG GTG CCG TCC AGC-3′ | 290-310 | ||
| UGT1A8 | 1A8-S | 5′-GGT CTT CGC CAG GGG AAT AG-3′ | 498-517 | 58 |
| 1A-AS | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 898-920 | ||
| UGT1A9 | 1A9-S | 5′-GAA CAT TTA TTA TGC CAC CG-3′ | 646-665 | 58 |
| 1A-AS | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 898-920 | ||
| UGT1A10 | 1A10-S | 5′-CTC TTT CCT ATG TCC CCA ATG A-3′ | 557-578 | 58 |
| 1A-AS | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 898-920 | ||
| UGT2B4 | 2B4-S | 5′-CAT CTT CAG CTT CCA TTT C-3′ | 170-188 | 52 |
| 2B4-AS | 5′-TCC TTA CAG AAC TTT CTA AG-3′ | 367-386 | ||
| UGT2B7 | 2B7-Sb | 5′-AGT TGG AGA ATT TCA TCA TGC AAC AGA-3′ | 254-280 | 58 |
| 2B-AS-1b | 5′-TCA GCC AGC AGC TCA CCA CAG GG-3′ | 463-485 | ||
| UGT2B10 | 2B10-Sb | 5′-TGA CAT CGT TTT TGC AGA TGC TTA-3′ | 432-455 | 62 |
| 2B-AS-2b | 5′-CAG GTA CAT AGG AAG GAG GGA A-3′ | 562-583 | ||
| UGT2B11 | 2B11-S | 5′-CTT CCA TTC TTT TTG ATC CCA ATG AT-3′ | 179-204 | 58 |
| 2B11-AS | 5′-GGA GAC TGT ACA CAA ACC-3′ | 500-517 | ||
| UGT2B15 | 2B15-Sb | 5′-GTG TTG GGA ATA TTA TGA CTA CAG TAA C-3′ | 348-375 | 62 |
| 2B-AS-1b | 5′-TCA GCC AGC AGC TCA CCA CAG GG-3′ | 466-488 | ||
| UGT2B17 | 2B17-Sb | 5′-GTG TTG GGA ATA TTC TGA CTA TAA TAT A-3′ | 348-375 | 58 |
| 2B-AS-2b | 5′-CAG GTA CAT AGG AAG GAG GGA A-3′ | 568-589 | ||
| UGT2B28 | 2B28-Sb | 5′-ATC CCA ATG ACG CAT TCA CTC TTA AAC TC-3′ | 194-222 | 62 |
| 2B-AS-2b | 5′-CAG GTA CAT AGG AAG GAG GGA A-3′ | 565-586 | ||
| GAPDH | hGAPDH-Sd | 5′-CCA GGG CTG CTT TTA ACT C-3′ | 56-74 | 52 |
|
| hGAPDH-ASd | 5′-GCT CCC CCC TGC AAA TGA-3′ | 330-347 |
|