Kinetic parameters of UGT activities in liver microsomes from male and female mice and rats
TFP N-glucuronide was not detected in liver microsomes from male and female mice and rats.
Substrate | Species | Sex | Km (S50) | Vmax | Ksi | CLint (CLmax) | n a |
|---|---|---|---|---|---|---|---|
| μM | nmol/min/mg protein | μM | μl/min/mg protein | ||||
| Estradiol | Mouse | M | 17 ± 3b | 6.1 ± 0.3 | 179c | 2.3 | |
| F | 18 ± 2b | 6.6 ± 0.4 | 183c | 2.3 | |||
| Rat | M | 16 ± 3b | 6.1 ± 0.4 | 193c | 1.8 | ||
| F | 16 ± 2b | 7.1 ± 0.3 | 231c | 1.8 | |||
| 4-MU | Mouse | M | 71 ± 8b | 214 ± 9 | 1975c | 1.2 | |
| F | 59 ± 3b | 159 ± 3 | 1436c | 1.5 | |||
| Rat | M | 130 ± 11b | 264 ± 11 | 1146c | 1.4 | ||
| F | 130 ± 6b | 286 ± 7 | 1241c | 1.3 | |||
| 4-NP | Mouse | M | 116 ± 23 | 40 ± 2 | 341 | ||
| F | 112 ± 24 | 33 ± 2 | 293 | ||||
| Rat | M | 378 ± 73 | 81 ± 7 | 213 | |||
| F | 635 ± 108 | 73 ± 6 | 115 | ||||
| Propofol | Mouse | M | 31 ± 3 | 1.9 ± 0.1 | 1069 ± 96 | 62 | |
| F | 51 ± 11 | 2.5 ± 0.3 | 514 ± 105 | 49 | |||
| Rat | M | 16 ± 4 | 0.22 ± 0.07 | 14 | |||
| F | 51 ± 21 | 0.06 ± 0.01 | 1.2 | ||||
| MPA | Mouse | M | 307 ± 59 | 24 ± 2 | 78 | ||
| F | 242 ± 21 | 27 ± 1 | 111 | ||||
| Rat | M | 344 ± 26 | 5.9 ± 0.2 | 17 | |||
| F | 304 ± 24 | 3.0 ± 0.1 | 9.9 | ||||
| Morphine | Mouse | M | 421 ± 17 | 19 ± 0 | 46 | ||
| F | 318 ± 15 | 20 ± 0 | 64 | ||||
| Rat | M | 1406 ± 17 | 39 ± 0 | 27 | |||
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| F | 1319 ± 79 | 35 ± 1 |
| 26 |
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