Effect of different ABC transport inhibitors on amounts of hesperetin 7-O-glucuronide and hesperetin 7-O-sulfate in the apical and basolateral medium 120 min after addition of 10 μM hesperetin at the apical side of Caco-2 monolayers compared with the control (10 μM hesperetin without inhibitors)
Mean ± S.D. values (n = 4, control n = 7).
Inhibitor | Hesperetin 7-O-Glucuronide | Hesperetin 7-O-Sulfate | |||||
|---|---|---|---|---|---|---|---|
| Apical | Basolateral | Apical | Basolateral | ||||
| nmol | |||||||
| Control | 1.71 ± 0.44 | 0.44 ± 0.12 | 0.25 ± 0.10 | 0.08 ± 0.05 | |||
| MK571 | |||||||
| 2.4 μM | 1.68 ± 0.42 | 0.41 ± 0.09 | 0.27 ± 0.06 | 0.09 ± 0.05 | |||
| 24 μM | 1.39 ± 0.44 | 0.46 ± 0.15 | 0.32 ± 0.06 | 0.12 ± 0.08 | |||
| CsA | |||||||
| 10 μM | 1.66 ± 0.43 | 0.53 ± 0.12 | 0.29 ± 0.09 | 0.11 ± 0.06 | |||
| PSC-833 | |||||||
| 5 μM | 1.70 ± 0.31 | 0.48 ± 0.10 | 0.32 ± 0.09 | 0.09 ± 0.05 | |||
| GF120918 | |||||||
| 1 μM | 1.39 ± 0.28 | 0.71 ± 0.17* | 0.17 ± 0.03 | 0.10 ± 0.04 | |||
| 5 μM | 1.11 ± 0.25* | 0.85 ± 0.30* | 0.16 ± 0.03 | 0.14 ± 0.08 | |||
| 10 μM | 1.09 ± 0.34* | 0.88 ± 0.17*** | 0.14 ± 0.05 | 0.14 ± 0.09 | |||
| Ko143 | |||||||
| 1 μM | 1.50 ± 0.23 | 0.82 ± 0.12*** | 0.23 ± 0.05 | 0.16 ± 0.04* | |||
| 2 μM | 0.89 ± 0.31** | 1.14 ± 0.40** | 0.14 ± 0.06 | 0.25 ± 0.11** | |||
| 5 μM | 0.96 ± 0.13** | 1.34 ± 0.18*** | 0.10 ± 0.04* | 0.26 ± 0.09** | |||