Primer and probe sequences used for measurements of steady-state mRNA levels in rat liver by real-time quantitative RT-PCR
CYP2C11 | β-Actin | |
|---|---|---|
| Forward primer | 5′-1219-TTTGACCCTGGTCACTTTCT-1238-3′ | 5′-357-GACCCAGATCATGTTTGAGACCTTC-381-3′ |
| Reverse primer | 5′-1318-GGGCTTCTCCTGCACATATC-1299-3′ | 5′-465-GGAGTCCATCACAATGCCAGTG-444-3′ |
| Probe | 5′-1272-CTCTTTCCTGCTGAGAATGGCATAAAG-1298-3′ | 5′-441-ACGACCAGAGGCATACAGGGACAACACAG-413-3′ |
| Product size | 100 base pairs | 109 base pairs |
| Reference | New design |
Tijet et al., 2006
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