TABLE 1

Summary of LC/MS/MS analytical conditions for selective P450 probe substrate metabolites measured from secondary incubations

Metabolitea
Electrospray Ionization Mode
MS Transition m/z
Gradient Profile %B(min)b
Acetaminophen + 152/110 10(0):10(1.2):98(3.5)
7-Hydroxycoumarin - 161/133 5(0):5(0.8):95(1.5)
Hydroxybupropion + 256/131 5(0):5(0.8):98(2.5)
6α-Hydroxypaclitaxel + 870/286 5(0):5(0.8):98(2.5)
4′-Hydroxydiclofenac - 310/266 5(0):5(0.3):95(2.5)
4′-Hydroxymephenytoin - 233/190 5(0):5(0.3):100(3.5)
Dextrorphan + 258/157 10(0):10(0.8):95(2.0)
6-Hydroxychlorzoxazone - 184/120 5(0):5(1.0):100(2.0)
1′-Hydroxymidazolam
+
342/203
10(0):10(0.8):95(2.0)

  • ↵ a Tolbutamide was used as the internal standard for both negative and positive ionization modes; the respective transitions were m/z 269/170 and 271/91.

  • ↵ b Mobile phases for all analyses were as follows: A, 0.1% formic acid in H2O and B, 0.1% formic acid in acetonitrile.