CYP4V2-dependent metabolism of saturated fatty acids
| Fatty acid | Metabolite* | Km | Vmax | Vmax/Km | ω/ω-1*** |
|---|---|---|---|---|---|
| μM | nmol/nmol/min | μl/nmol/min | |||
| Octanoic acid | 8-OH octanoate | —** | <0.1 | —** | —** |
| Lauric acid | 12-OH laurate | 140 ± 20 | 3.7 ± 0.27 | 26 | >20:1 |
| Myristic acid | 14-OH myristate | 65 ± 7.4 | 2.3 ± 0.07 | 35 | >20:1 |
| Palmitic acid | 16-OH palmitate | 430 ± 89 | 3.5 ± 0.26 | 8.1 | >10:1 |
↵* ω-Hydroxy metabolites of the C8 to C16 substrates were detected as the molecular ions of the TMS derivatives at m/z 289, 345, 373, and 401, respectively.
↵** Reaction velocities were below quantifiable levels.
↵*** Regioselectivity was determined by GC-FID analysis of the TMS derivatives, assuming an equal detector response for the ω and ω-1 metabolites.