TABLE 2

Androsterone and etiocholanolone glucuronidation kinetics

The values represent a best-fit result ± S.E. The reaction velocity is normalized to UGT2B17 expression level.

Androsterone
Etiocholanolone
VmaxKmVmaxKm
pmol/mg/min μM pmol/mg/min μM
UGT2A1Not determinedNot determined4919 ± 701249 ± 48
UGT2B7246 ± 14a4.3 ± 0.665 ± 1.65.4 ± 0.5
UGT2B17280 ± 50.9 ± 0.1814 ± 43b1.3 ± 0.3

  • ↵a Data fitted to substrate inhibition equation, Ksi 658 ± 333 μM.

  • ↵b Data fitted to substrate inhibition equation, Ksi 494 ± 211 μM.