Absolute concentrations of the hydroxylated metabolites detected after multiple administration (day 1-day 2-day 3) of AD in four nonchimeric mice (Non-Chim1—Non-Chim4)
| Concentrations (n = 3) | ||||||
|---|---|---|---|---|---|---|
| 6α OH-AD | 4OH-AD | 16α OH-AD | 6β OH-A | 6β OH-E | 16α OH-A | |
| ng/ml ± RSD% | ||||||
| Non-Chim1 | ||||||
| Day 1 | 36.1 ± 6.5 | 65.4 ± 0.5 | 249.8 ± 8.0 | 85.7 ± 2.8 | 27.7 ± 2.1 | 77.6 ± 2.3 |
| Day 2 | 46.9 ± 4.1 | 88.3 ± 4.0 | 299.3 ± 7.8 | 141.6 ± 4.1 | 76.5 ± 0.6 | 108.6 ± 3.4 |
| Day 3 | 77.7 ± 2.6 | 177.1 ± 7.5 | 495.0 ± 11.0 | 214.5 ± 10.9 | 100.2 ± 9.1 | 180.2 ± 8.7 |
| Non-Chim2 | ||||||
| Day 1 | 19.0 ± 13.4 | 78.8 ± 2.0 | 78.4 ± 4.8 | 202.2 ± 3.1 | 81.9 ± 7.1 | |
| Day 2 | 31.5 ± 1.5 | 64.6 ± 15.4 | 85.4 ± 11.3 | 248.7 ± 12.8 | 57.0 ± 6.4 | |
| Day 3 | 26.3 ± 3.5 | 67.0 ± 10.1 | 90.3 ± 4.9 | 148.1 ± 17.6 | 50.2 ± 15.2 | |
| Non-Chim3 | ||||||
| Day 1 | 19.5 ± 5.3 | 33.7 ± 4.5 | 83.7 ± 11.3 | 105.4 ± 5.2 | 94.3 ± 6.1 | 101.9 ± 9.2 |
| Day 2 | 29.2 ± 8.9 | 47.9 ± 3.2 | 96.8 ± 2.2 | 79.9 ± 2.9 | 71.7 ± 2.5 | 84.0 ± 4.0 |
| Day 3 | 25.3 ± 4.8 | 57.8 ± 2.4 | 216.5 ± 6.0 | 80.6 ± 3.6 | 79.4 ± 2.3 | 92.4 ± 9.4 |
| Non-Chim4 | ||||||
| Day 1 | 18.4 ± 5.3 | 15.2 ± 4.4 | 73.2 ± 3.3 | 73.0 ± 13.4 | 50.6 ± 14.9 | 71.9 ± 4.3 |
| Day 2 | 35.3 ± 2.0 | 54.6 ± 0.7 | 86.7 ± 13.4 | 158.4 ± 7.7 | 92.4 ± 5.7 | 145.5 ± 1.2 |
| Day 3 | 38.3 ± 2.7 | 68.0 ± 0.9 | 162.1 ± 0.7 | 157.9 ± 6.6 | 105.0 ± 3.3 | 132.2 ± 1.3 |
6αOH-AD, 6α-hydroxyandrostenedione; 4OH-AD, 4-hydroxyandrostenedione; 16αOH-AD, 16α-hydroxyandrostenedione; 6β OH-A, 6β-hydroxyandrosterone; 6β OH-E, 6β-hydroxyetiocholanolone; 6αOH-A, 16α-hydroxyandrosterone.