R = | KMa | Vmaxb | V/Kc | KIEd |
---|---|---|---|---|
μ M | nmol/s/mg enzyme | |||
OCH3 | 85 | 9 | 101 | N.D. |
CH3 | 56 | 121 | 2161 | 5.2 |
H | 31 | 17 | 548 | N.D. |
Cl | 11 | 164 | 14,909 | 3.0 |
N.D., not determined.
↵a The concentration at half-maximal velocity. Errors are approximately 10% for each value.
↵b The maximum velocity estimated from the asymptote for the hyperbola. Errors are approximately 10% for each value.
↵c V/K multiplied by 1000.
↵d The isotope effect on V/K determined by taking the ratio of V/K for the proton/deuterium substitution at the site of oxidation from Skibo et al. (1987).