Primer sequence used for real-time RT-PCR quantification and for calibrator preparation
Isoform | F Primer | R Primer | Amplicon Size |
|---|---|---|---|
| bp | |||
| For calibrator preparation | |||
| 1A1 | 5′-AATAAAAAAGGACTTGCTATGCT-3′ | 562* | |
| 1A3 | 5′-TGTTGAACAATATGTTTTGGTCTA-3′ | 562* | |
| 1A4 | 5′-GAACAATGTATCTTTGCCC-3′ | 558* | |
| 1A5 | 5′-TATGAACAATATGTCTTGATCATACA-3′ | 561* | |
| 1A6 | 5′-CATGATTGTTATTGGCTGTAC-3′ | 558* | |
| 1A7 | 5′-GCGAAGTGCATTTTCCTATTAAC-3′ | 591* | |
| 1A8 | 5′-GAAAGCACAAGTACAAGTTTG-3′ | 603* | |
| 1A9 | 5′-TGGAAAGCACAAGTCGAAGTATATA-3′ | 605* | |
| 1A10 | 5′-GAAAGCACAGGCACAAGTATA-3′ | 603* | |
| Common primer | 5′-ATTCCATGTTTCCAGAAGCA-3′ | ||
| For quantification | |||
| 1A1 | 5′-AATAAAAAAGGCTCTGCTATGCT-3′ | 5′-ACATCAAACTGCTTTCTGC-3′ | 96 |
| 1A3 | 5′-TGTTGAACAATAGTCTTTGGTCTA-3′ | 5′-ACCACATCAAGGAAGTAGCA-3′ | 100 |
| 1A4 | 5′-GAACAATGTATCTTGGCCC-3′ | 5′-ACCACATCAAGGAAGTAGCA-3′ | 96 |
| 1A5 | 5′-TATGAACAATATTCTTTGATCATACA-3′ | 5′-ACCACATCAAGGAAGTAGCA-3′ | 99 |
| 1A6 | 5′-CATGATTGTTATGGCCTGTAC-3′ | 5′-TCTGTGAAAGAGCATCAAACT-3′ | 105 |
| 1A7 | 5′-GCGAAGTGCATTTCTCTATTAAC-3′ | 5′-GGGAGGGAAAATATTTGGC-3′ | 186 |
| 1A8 | 5′-GAAAGCACAAGACGAAGTTTG-3′ | 5′-GGGAGGGAAAATATTTGGC-3′ | 198 |
| 1A9 | 5′-TGGAAAGCACAGTACGAAGTATATA-3′ | 5′-GGGAGGGAAAATATTTGGC-3′ | 200 |
| 1A10 | 5′-GAAAGCACAGGACAAAGTATA-3′ | 5′-GGGAGGGAAAATATTTAGCAAC-3′ | 198 |
| For quantification and calibrator preparation | |||
| 2B4 | 5′-TCTTTCGATCCAACAGCC-3′ | 5′-CATCTCTTAACCGCTGCTTGATA-3′ | 104 |
| 2B7 | 5′-GGAGAATTTCTCATGCAACAGA-3′ | 5′-CAGAACTTTCTATTATGTCACCAAATATTG-3′ | 123 |
| 2B10 | 5′-ATTTGAGAATTCATCATGCAAT-3′ | 5′-ATGTCATTAATTCCCACAG-3′ | 111 |
| 2B11 | 5′-TCAGACATTCAAAAGATAGC-3′ | 5′-ATCTTTACAGAATTTCTAAATATGTCATAT-3′ | 96 |
| 2B15 | 5′-CTTCTGAAAATCTCGATAGATGGAT-3′ | 5′-CATCTTTACAGACTTGTTACTGTAGTCAT-3′ | 124 |
| 2B17 | 5′-TTTATGAAAAGTTCGATAGATGGAC-3′ | 5′-CATCTTCACAGACTTTATATTATAGTCAG-3′ | 124 |
| For calibrator preparation | |||
| GAPDH | 5′-ATGGGGAAGTGAAGGTCG-3′ | 5′-TTACTCCTTGAGGCCATGT-3′ | 1008 |
| For quantification | |||
| GAPDH | 5′-TCCACTGGCTCTTCACC-3′ | 5′-GGCAGAGATATGACCCTTTT-3′ | 78 |
F, forward; R, reverse.
↵* Amplicon Size with common R primer.