Canine rP450 Bactosome kinetic parameters
Isoform | Substrate/Metabolite | V1 a | Km1 | V2 | Km2 |
|---|---|---|---|---|---|
| nmol/min/nmol | μM | nmol/min/nmol | μM | ||
| CYP1A1 | Phenacetin/4-acetamidophenol | 10.5 ± 0.1 | 49.3 ± 2.0 | NA | NA |
| CYP2B11 | Temazepam/oxazepam | 2.1 ± 0.9 | 4.7 ± 4.4 | 20.6 ± 2.8 | 374 ± 147 |
| CYP2C21 | Diclofenac/4′-OH | 12.9 ± 0.93 | 50.1 ± 14.0 | NA | NA |
| CYP2C41 | Diclofenac/4′-OH | 4.6 ± 0.7 | 569 ± 165 | NA | NA |
| CYP2D15 | Bufuralol/1′-OH | 11.3 ± 0.2 | 9.4 ± 0.6 | NA | NA |
| CYP3A12 | Midazolam/1′-OH | 0.84 ± 0.02 | 3.8 ± 0.4 | NA | NA |
| CYP3A26 | Midazolam/1′-OH | 0.074 ± 0.021 | 2.0 ± 1.3 | 0.30 ± 0.03 | 120 ± 53 |
| CYP3A12 | Midazolam/4-OH | 0.56 ± 0.02 | 37 ± 3.2 | NA | NA |
| CYP3A26 | Midazolam/4-OH | 0.073 ± 0.040 | 0.83 ± 1.4 | 0.50 ± 0.04 | 62.3 ± 23.7 |
NA, Not applicable.
↵ a See Materials and Methods for description of experiments and kinetic models.