LC/MS analysis of benzphetamine metabolism by P450 2B6
The incubations, metabolite extraction, and analysis by LC/MS were described under Materials and Methods. Quantitation was performed in the presence of an internal standard (d3-norbenzphetamine), and the results were normalized to the control (native) sample as 100%.
Metabolite | Area under the Peak | Percentage of Native | ||
|---|---|---|---|---|
| Native | Inactivated | |||
| OH-benzphetamine | 79 ± 6 | 57 ± 17 | 72 | |
| OH-norbenzphetamine | 7.0 ± 2 | 2.2 ± 1 | 31* | |
| Norbenzphetamine | 63 ± 0.7 | 16 ± 10 | 25* | |
| Methamphetamine | 2.3 ± 2 | 1.7 ± 1 | 74 | |
| Amphetamine | 1.2 ± 1 | 0.7 ± 0.5 | 58 | |
↵* , indicates statistical significance; P < 0.05.