Effects of inactivation by PCP on the rates of NADPH oxidation, product formation, and hydrogen peroxide production by P450 2B6
The incubations, assays, and analyses are described under Materials and Methods. The values represent the mean and S.D. of three determinations done in duplicate.
P450 2B6 | EB | NADPH | 1-PhE | H2O2 | Ratio of NADPH/1-PhE/H2O2 | Activity Remaininga |
|---|---|---|---|---|---|---|
| nmol/min/nmol P450 | nmol/min/nmol P450 | nmol/min/nmol P450 | % | |||
| Native | - | 28 ± 4 | - | 26 ± 1 | 1.0:0.0:0.9 | 100 |
| Native | + | 81 ± 2 | 54 ± 4 | 25 ± 1 | 1.0:0.7:0.3 | 100 |
| Inactivated | + | 59 ± 7 | 20 ± 4 | 46 ± 2 | 1.0:0.3:0.7 | 35 |
1-PhE, 1-phenylethanol.
↵ a The percentage activity remaining was determined from the 7-EFC O-deethylation assay as described under Materials and Methods.