TABLE 5

Uptake clearance of drugs in rat kidney slices

Time-dependent uptake of drugs into rat kidney slices was measured in the presence of tracer amount of drugs in the incubation buffer (0.1 μM for [³H]pravastatin, [³H]pitavastatin, [³H]rosuvastatin, [³H]valsartan, and [³H]olmesartan; 6.3 μM for [¹⁴C]temocaprilat; 1.7 μM for [¹⁴C]PCG; 10 μM for enalaprilat, benazeprilat, cefmetazole, and ceftizoxime). The in vitro intrinsic uptake clearance (P_{1 kid}) was calculated by dividing initial uptake velocity by the drug concentration in the incubation buffer. Each value is expressed as mean ± S.E. of triplicate determinations.