Primers used in the present study
Primer | Sequence |
|---|---|
| For detection of real-time RT-PCR | |
| AADAC-RT-S | 5′-TTGTGGAGCTCCTGGGACTT-3′ |
| AADAC-RT-AS | 5′-TCTGTCTGCTGTCCATCTTG-3′ |
| For construction of expression plasmids | |
| AADAC-S | 5′-TAGAGACCAAGAAGCGGGAC-3′ |
| AADAC-AS | 5′-GCTACATGTTTTACTATAGATTTTCC-3′ |
| CES1A1-S | 5′-AGAGACCTCGCAGGCCCCGA-3′ |
| CES1A2-S | 5′-GAGACCTCGCAGGCCCCCG-3′ |
| CES1A-AS | 5′-CCATGGTAAGATGCCTTCTG-3′ |
| CES2A1-S | 5′-CCTGCCTACCACTAGATCCC-3′ |
| CES2A1-AS | 5′-CTCGCCTGTCAGCGAACCCAC-3′ |