TABLE 1
Properties of ketamine and metabolites that were monitored in microsomal preparations and in urine
Retention times and m/z ratios targeted for the metabolites of ketamine showing all the isomers of hydroxynorketamine and hydroxyketamine are included. Accurate masses are shown where obtained (with ppm error from theoretical).
Structure | Abbreviation | LC tR | m/z (Deuterated Analog) | Measured m/z (ppm) |
|---|---|---|---|---|
| min | ||||
| K | 3.7 | 238 (242) | 238.09923 (0.084) |
| NK | 3.5 | 224 (228) | 224.08365 (0.089) |
| DHNK | 3.0 | 222 (226) | 222.06801 (0.045) |
| HK 1 | 2.0 | 254 (258) | 254.09418 (0.275) |
| HK 2 | 2.6 | 254 (258) | 254.09418 (0.275) | |
| HK 5 | 5.8 | 254 (258) | —a | |
| HK 3 | 3.2 | 254 (257) | 254.09418 (0.275) |
| HK 4 | 3.4 | 254 (257) | — | |
| HNK 1 | 1.7 | 240 (244) | 240.07858 (0.042) |
| HNK 2 | 2.2 | 240 (244) | 240.07857 (0.042) | |
| HNK 3 | 2.4 | 240 (244) | 240.07858 (0.042) | |
| HNK 7 | 5.8 | 240 (244) | 240.07858 (0.042) | |
| Glucuronide conjugate | 5.3 | 416 (420) | 416.11075 (0.192) | |
| HNK 4 | 2.8 | 240 (243) | 240.07863 (0.208) |
| HNK 5 | 3.1 | 240 (243) | 240.07858 (0.042) | |
| HNK 6 | 3.3 | 240 (243) | 240.07870 (0.499) | |
|
| Glucuronide conjugate | 1.2 | 416 (419) | — |
-
↵ a —, insufficient metabolite for accurate mass determination.