TABLE 2

Sequence of primers used for RT-PCR analyses

Primer
Sequence
Position
1A4 ex1-Sa 5′-ACG CTG GGC TAC ACT CAA GG-3′ 277-296b
1A4 ex1-ASa 5′-TCT GAA TTG GTC GTT AGT AAC T-3′ 587-608b
1A5 ex1-Sa 5′-ACA ATA TGT CTT TGA TCA TA-3′ 353-372b
1A5 ex1-ASa 5′-AGA AAC AGC ATG GCA AAG-3′ 667-684b
1A ex2-ASa 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ 910-932b
int-S 5′-CAA GGA TAA TTC TGT AAG GCA-3′ IVS2-221 – IVS2-201c
int-ASd
5′-CAT TGA TTG GAT GAA GGC AC-3′
IVS1 + 11 – IVS1 + 30c

  • ↵ a Nakamura et al. (2008b).

  • ↵ b Nucleotide position on cDNA when the A in the start codon is 1.

  • ↵ c Nucleotide position from the nearest exon on genome DNA.

  • ↵ d This primer can anneal both UGT1A4 and UGT1A5.