Characterization of insect cell microsomes coexpressing CYP2C9 and NADPH-cytochrome P450 oxidoreductase
Data are presented as the mean ± S.D. of three to four different expression experiments.
Recombinant Enzymes (Amino Acid Alteration) | P450 Amount | OR Activity | Molar Ratio (OR/P450) |
|---|---|---|---|
| pmol of P450/mg of protein | nmol of cytochrome c reduced/min/mg of protein | ||
| CYP2C9.1 (wild type) | 191 ± 19 | 686 ± 47 | 1.21 ± 0.14 |
| CYP2C9.3 (I359L) | 190 ± 26 | 586 ± 77 | 1.06 ± 0.26 |
| CYP2C9.13 (L90P) | 22 ± 5*** | 608 ± 7 | 9.63 ± 2.43 |
| CYP2C9.26 (T130R) | 158 ± 27 | 614 ± 205 | 1.31 ± 0.47 |
| CYP2C9.28 (Q214L) | 165 ± 31 | 674 ± 32 | 1.40 ± 0.27 |
| CYP2C9.30 (A477T) | 201 ± 34 | 675 ± 86 | 1.16 ± 0.30 |
| CYP2C9.33 (R132Q)a | 192 ± 15 | 758 ± 43 | 1.32 ± 0.03 |
| CYP2C9.34 (R335Q)a | 159 ± 5 | 748 ± 29 | 1.56 ± 0.03 |
↵ a Data on CYP2C9.33 and CYP2C9.34 was reported previously (Yin et al., 2008).
↵*** P < 0.001 vs. wild type. One-way ANOVA with a post hoc Dunnett multiple comparisons test.