Comparison of the polarized distribution and relative expression at mRNA/protein level (immunohistochemistry, Western blot, and functionality) of placental transporters relevant to drug disposition across the maternal-fetal interface
| Localization | Transporter | Placenta | BeWo | Jeg-3 | JAR | Reference |
|---|---|---|---|---|---|---|
| Brush border (maternal facing) membrane | P-gp (MDR1) | ++/+ | +/+ | +/+ | ++/+ | St-Pierre et al., 2000; Patel et al., 2003; Evseenko et al., 2006 |
| Pascolo et al., 2000; St-Pierre et al., 2000; Evseenko et al., 2006; Serrano et al., 2007; Prouillac et al., 2009 | ||||||
| MRP2 | +/+ | ++/+ | +/nd | ++/− | Serrano et al., 2007 | |
| Maliepaard et al., 2001; Evseenko et al., 2006; Yeboah et al., 2006 | ||||||
| MRP8 | +/nd | +/nd | ++/nd | +/nd | Patel et al., 2003; Ugele et al., 2003; Serrano et al., 2007 | |
| BCRP | +/+ | ++/++ | +/+ | ++/+ | Patel et al., 2003; Ugele et al., 2003; Serrano et al., 2007 | |
| Patel et al., 2003; Ugele et al., 2003; Serrano et al., 2007 | ||||||
| OATP1A2 (OATP-A) | +/nd | +/nd | +/nd | +/nd | Sato et al., 2003 | |
| OATP1B1 (OATP-C) | +/nd | nd/nd | nd/nd | nd/nd | Tamai et al., 1997; Ganapathy et al., 2005 | |
| OATP3A1 (OATP-D) | +/nd | nd/nd | nd/nd | nd/nd | Lahjouji et al., 2004; Rytting and Audus, 2005, 2007 | |
| OATP4A1 (OATP-E) | nd/+ | nd/nd | nd/nd | nd/nd | Wang et al., 2000 | |
| OCTN1/SLC22A4 | +/+ | nd/nd | nd/nd | nd/nd | Barros et al., 1995; Yasuda et al., 2008 | |
| OCTN2/SLC22A5 | +/+ | +/+ | nd/nd | nd/nd | Settle et al., 2004 | |
| NaDC3 | nd/+ | nd/nd | nd/nd | nd/nd | Yasuda et al., 2008; Solanky et al., 2009 | |
| hENT1/SLC29A1 | nd/+ | +/+ | nd/nd | nd/nd | Yasuda et al., 2008; Solanky et al., 2009 | |
| MCT-4/SLC16A4 | +/+ | nd/nd | nd/nd | nd/nd | Yasuda et al., 2008; Solanky et al., 2009 | |
| FRα | +/+ | +/+ | nd/nd | +/nd | Prasad et al., 1996 | |
| PCFT (SLC46A1) | +/+ | +/nd | nd/nd | nd/nd | Bzoskie et al., 1997 | |
| RFC-1 (SLC19A1) | +/+ | +/nd | nd/nd | nd/nd | ||
| SERT | +/+ | +/+ | nd/nd | +/+ | ||
| NET | +/+ | nd/nd | nd/nd | nd/nd | ||
| Basal (fetal facing) membrane | MDR3 | +/++ | +a/+ | +a/nd | +a/++ | Patel et al., 2003; Evseenko et al., 2006 |
| MRP5 | ++/+ | +a/nd | nd/nd | nd/nd | Pascolo et al. 2003; Meyer zu Schwabedissen et al., 2005 | |
| OATP2B1 (OATP-B) | ++/++ | +/nd | +/nd | +/nd | St-Pierre et al., 2002; Grube et al., 2007; Serrano et al., 2007 | |
| OAT4 | ++/+ | nd/nd | nd/nd | nd/nd | Cha et al., 2000; Ugele et al., 2003 | |
| RFC-1 (SLC19A1) | +/+ | +/+ | nd/nd | nd/nd | Yasuda et al., 2008; Solanky et al., 2009 | |
| OCT3 | +/+ | −/nd | nd/nd | nd/nd | Sata et al., 2005 | |
| MCT-1/SLC16A1 | +/+ | nd/nd | nd/nd | nd/nd | Settle et al., 2004 | |
| Unknown or controversial | MRP1 | +/+ | ++/++ | ++/+ | +/+ | St-Pierre et al., 2000; Nagashige et al., 2003; Evseenko et al., 2006 |
| localization | St-Pierre et al., 2000 | |||||
| MRP3 | ++/+ | +/nd | +/nd | +/nd | Serrano et al., 2007 | |
| MRP4 | +/nd | +/nd | ++/nd | +/nd | Utoguchi et al., 1999; Chan et al., 2006 | |
| MCT-8 | +/+ | nd/+ | nd/nd | nd/nd | Price et al., 1998; Settle et al., 2004 | |
| MCT3, MCT5, MCT7 | +/nd | nd/nd | nd/nd | nd/nd | Barros et al., 1995 | |
| hENT2/SLC29A2 | nd/+ | nd/nd | nd/nd | nd/nd |
nd, no data available.
↵a limit detection.
+, positive signal.
++, strong positive signal.
−, negative result.