Primers used for site-directed mutagenesis
Mutated bases are in boldface and underlined. Reverse primers were complementary to the forward primers.
Mutant | Template | Forward Primer |
|---|---|---|
| 1A1 (H39P) | UGT1A1 | 5′-CCCAGTGGATGGCAGCCCCTGGCTGAGCATGCTTG-3′ |
| 1A6 (H38P) | UGT1A6 | 5′-CCCTCAGGACGGAAGCCCCTGGCTTAGTATGAAGG-3′ |
| 1A9 (H37P) | UGT1A9 | 5′-CCCATGGATGGGAGCCCCTGGTTCACCATGAGG-3′ |
| 2B7 (H35P) | UHT2B7 | 5′-GGGCAGCAGAATACAGCCCTTGGATGAATATAAAGAC-3′ |
| 2B10 (L34H) | UGT2B10 | 5′-GGGCCGCAGAATACAGCCATTGGATGAATATGAAGAC-3′ |