Mean enzyme kinetic parameters for glucuronide conjugate formation from 1′- and 4-hydroxymidazolam in HLMs
The values are the means of estimates from three different HLM preparations.
| Kinetic Parameter | ||||
|---|---|---|---|---|
| Km | Vmax | Hill Coefficient (n) | CLint (Vmax/Km) | |
| μM | pmol/(min · mg protein) | μl/(min · mg protein) | ||
| 1′-Hydroxymidazolam | ||||
| N-Glucuronide | 9.1 | 790.0 | 1.6 | 86.8 |
| O-Glucuronide | 12.7 | 3285.8 | 1.5 | 259.4 |
| 4-Hydroxymidazolam | ||||
| Glucuronide I | 41.5 | 3609.9 | 1.2 | 87.0 |
| Glucuronide II | 976.9 | 3210.5 | 0.5 | 3.3 |