TABLE 3

Turnover rates for midazolam in suspended rat hepatocytes and hepatic microsomes

Midazolam conc.Hepatocellular Rate of FormationMicrosomal Rate of Formation
1′-OH-midazolam4-OH-midazolamRatio 4-OH/1′-OH1′-OH-midazolam4-OH-midazolamRatio 4-OH/1′-OH
pmol/min/g liver
5 μM5.3 ± 1.312.7 ± 4.22.47.6 ± 2.819.9 ± 7.62.6
15 μM9.9 ± 1.525.4 ± 6.92.69.0 ± 4.133.9 ± 12.82.9
30 μM11.2 ± 1.429.6 ± 7.92.612.5 ± 4.337.2 ± 12.92.9