Enzyme | Detergenta | kcat | Km | kcat/Km |
---|---|---|---|---|
min−1 | mM | min−1 mM−1 | ||
MBP-FMO3 | Minimal detergent | 7.0 ± 0.3 | 6.5 ± 0.8 | 1.1 ± 0.2 |
CHAPS | 8.6 ± 0.2 | 5.5 ± 0.5 | 1.6 ± 0.1 | |
Triton X-100 | 8.5 ± 0.2 | 4.1 ± 0.3 | 2.1 ± 0.2b | |
Supersomal FMO3 | Minimal detergent | 45c | N.D. | N.D. |
Triton X-100 | 47 ± 3d | N.D. | N.D. |
N.D., not determined.
↵a When present, detergent concentrations were 0.5% (w/v). Unless otherwise indicated, values are the fitting value and error from a single determination.
↵b Comparison of this value to that obtained with minimal detergent conditions yielded p = 1.3 × 10−3.
↵c The indicated value is based on a single determination at 50 mM methionine.
↵d The indicated value is the average and S.D. of two independent determinations at 50 mM methionine.