TABLE 2

Summary of Cyp2b10, Cyp2c29, and Cyp3a11 activity in db/db and control mice liver microsomes

Formation of resorufin, 4-OH-mephenytoin, and 4-OH-midazolam was measured to assess Cyp2b10, Cyp2c29, and Cyp3a11 metabolism, respectively. Data are depicted as the mean ± S.D.; n = 5.

Cyp2b10Cyp2c29Cyp3a11
10-Week db/db25-Week db/db25-Week Control10-Week db/db25-Week db/db25-Week Control10-Week db/db25-Week db/db25-Week Control
Vmax [nmol/(min · nmol P450)]0.0473 ± 0.0069††0.0697 ± 0.0115***0.0232 ± 0.00410.307 ± 0.0870.334 ± 0.034*0.203 ± 0.0601.03 ± 0.231.27 ± 0.061.23 ± 0.07
Km (μM)3.27 ± 0.503.78 ± 1.15*2.30 ± 0.44127 ± 48161 ± 34167 ± 316.04 ± 2.067.02 ± 2.488.36 ± 1.41
Vmax/Km [μl/(min · nmol P450)]14.5 ± 01.819.2 ± 4.3*10.2 ± 1.62.49 ± 0.00312.18 ± 0.63*1.22 ± 0.31179 ± 37196 ± 59150 ± 20
Vmax/Km [μl/(min · mg microsome)]8.02 ± 1.07††5.82 ± 0.76*4.29 ± 0.351.55 ± 0.35†††0.786 ± 0.042*0.444 ± 0.076103 ± 4071.3 ± 20.547.7 ± 23.6
  • * p < 0.05, significantly different from that of the 25-week-old control mice.

  • *** p < 0.001.

  • †† p < 0.01, significantly different from that of the 25-week-old db/db mice.

  • ††† p < 0.001.