TABLE 2

Inhibitory effects of FK1706 on midazolam 1′-hydroxylase activity in preincubation samples of human liver microsomes with or without NADPH and/or FK1706 and before and after dialysis

Data are expressed as mean ± S.D. (n = 3). Values in parentheses show the percentage of control.

FK1706 Conc. during PreincubationMidazolam 1′-Hydroxylase Activity
Before DialysisAfter Dialysis
NADPH (+)NADPH (−)NADPH (+)NADPH (−)
pmol/min/mg protein
0 μM (control)150 ± 10114 ± 954.1 ± 2.951.9 ± 2.9
2 μM9.94 ± 0.18 (6.6)60.9 ± 5.7 (53.5)3.48 ± 0.59 (6.4)50.3 ± 2.8 (96.9)