Apparatus and LC-MS/MS analytical conditions for each drug during the determination of concentration in cynomolgus monkey plasma
| LC System | MS/MS System | Column | Column Temperature | Injection Volume | Flow Rate | Mobile Phase | |
|---|---|---|---|---|---|---|---|
| °C | μl | ml/min | |||||
| Hydrochlorothiazide | HP1100 | API-2000 | Inertsil ODS 3.3 μM (2.1 × 50 mm) | 40 | 40 | 0.2 | 0.1% Formic acid: acetonitrile = 1:1 |
| Nifedipine | HP1100 | API-2000 | Inertsil ODS 3.5 μM (3.0 × 150 mm) | Room temperature | 30 | 0.2 | Water: acetonitrile = 4:6 |
| Quinidine | LC-VP/LC-10A | API-3000 | Xterra MS C18 (4.6 × 50 mm) | 40 | 10 | 0.3 | 20 mM Ammonium acetate (pH4.8): acetonitrile = 4:6 |
| Verapamil | LC-VP/LC-10A | API-3000 | Xterra MS C18 (4.6 × 50 mm) | 40 | 10 | 0.3 | 20 mM Ammonium acetate (pH4.8): acetonitrile = 4:6 |
| Digoxin | LC-VP/LC-10A | API-3000 | Xterra MS C18 (4.6 × 30 mm) | 40 | 20 | 0.3 | 2 mM Ammonium acetate: acetonitrile = 65:35 |
| Propranolol | LC-VP/LC-10A | API-3000 | Xterra MS C18 (4.6 × 50 mm) | 40 | 10 | 0.3 | 20 mM Ammonium acetate (pH4.8): acetonitrile = 4:6 |
| Amitriptyline | LC-VP/LC-10A | API-3000 | Xterra MS C18 (4.6 × 50 mm) | 40 | 10 | 0.3 | 20 mM Ammonium acetate (pH4.8): acetonitrile = 4:6 |
| Timolol | LC-VP/LC-10A | API-3000 | Xterra MS C18 (4.6 × 50 mm) | 40 | 10 | 0.3 | 20 mM Ammonium acetate (pH4.8): acetonitrile = 4:6 |
| Ibuprofen | LC-VP/LC-10A | API-3000 | Xterra MS C18 (4.6 × 50 mm) | 40 | 10 | 0.3 | 20 mM Ammonium acetate (pH4.8): acetonitrile = 4:6 |