TABLE 6

Michaelis-Menten kinetic parameters for amiodarone metabolism by HLM, HIM, recombinant CYP2J2, and recombinant CYP3A4 to N-desethyl amiodarone and hydroxy amiodarone by CYP3A4 and CYP2J2

KmVmaxVmax/Km
μMpmol/min/mg proteinμ l/min/mg protein
N-Desethyl amiodarone
    HLM7.41 ± 0.691009 ± 34136.2
    HIM11.48 ± 3.79342 ± 4229.8
    rCYP2J22.15 ± 0.461.05 ± 0.07a0.5b
    rCYP3A43.62 ± 0.4329.76 ± 1.10a8.2b
Hydroxy amiodarone
    HLM14.9 ± 2.60
    HIM5.11 ± 3.91
    rCYP2J23.89 ± 0.79
  • a Unit of measure is pmol/min/pmol rP450.

  • b Unit of measure is μl/min/pmol rP450.