TABLE 1

List of primers used for PCR amplifications of UGT1A10 wild-type and deletion promoter regions were designed with the addition of restriction enzyme sites at their 5′ proximal ends for vector insertion in the correct orientation

Primer Name^a	Restriction Site	Primer sequence (5′–3′)	Primer Location
WT186S	KpnI	5′-`AGTAGGTACCTCAGCAAATGATACT`-3′	−190 to −176^b
WT186AS	MluI	5′-`AGCCACGCGTGAACTGCAGCCCGAGCC`-3′	−21 to −5^b
WT441S	KpnI	5′-`CTGTGGTACCCCTGGAACATGAGATGCC`-3′	−445 to −428^b
WT441AS	MluI	5′-`AGCCACGCGTGAACTGCAGCCCGAGCC`-3′	−21 to −5^b
WT2204S	MluI	5′-`AGCCACGCGTGTATTAGGTTTGCTTGGT`-3′	−2208 to −2187^b
WT2204AS	XmaI	5′-`TATACCCGGGGAACTGCAGCCCGAGCC`-3′	−21 to −5^b
Del441S	KpnI	5′-`GCGGGCGGTACCTAAAGTCTCCCACTA`-3′	−445 to −431^c
Del441AS	MluI	5′-`AGCCACGCGTGAACTGCAGCCCGAGCC`-3′	−21 to −5^c
DEL 2204S	MluI	5′-`CGGCCACGCGTGAATGCTTGTG`-3′	−2208 to −2198^c
DEL 2204AS	XmaI	5′-`TATACCCGGGGAACTGCAGCCCGAGCC`-3′	−21 to −5^c

↵a Primer names with S or AS refer to sense and antisense primers, respectively.
↵b Relative to the UGT1A10 wild-type translation start site.
↵c Relative to the UGT1A10 deletion variant translation start site.