Effects of rifampicin siHNF4 versus scrambled siRNA and rifampin on CYP2C8, CYP2C9, and CYP2C19 mRNA in primary human hepatocytes infected with scrambled siRNA or siHNF4-I

Expression of CYP2C8, CYP2C9, CYP2C19, and HNF4α mRNAs was evaluated in primary human hepatocytes in triplicate from four different donors after infection with adenovirus containing scrambled siRNA or siHNF4-I in the presence of 0.2% DMSO or 10 μM rifampicin. Data are expressed as fold induction over the DMSO controls and represent the mean ± S.D. of the four donors. Values were compared using Mann-Whitney tests.

SCR1.00 ± 0.001.06 ± 0.111.00 ± 0.006.58 ± 1.79a1.00 ± 0.003.00 ± 0.70a1.00 ± 0.001.84 ± 0.31a
siHNF40.31 ± 0.09b0.40 ± 0.10a,b0.47 ± 0.06b1.09 ± 0.45a,b0.45 ± 0.14b0.63 ± 0.14b0.57 ± 0.15b0.66 ± 0.24b
  • SCR, scrambled; Rif, rifampin.

  • a Values from cells treated with rifampicin significantly higher than those treated with DMSO for cells infected with scrambled siRNA or siHNF4-I, p < 0.05.

  • b Values from cells treated with siHNF4-I significantly lower than those treated with SCR siRNA for either DMSO or rifampicin controls, p < 0.05.