TABLE 3

Comparison of fold induction of CYP2C8, CYP2C9, and CYP2C19 mRNAs by rifampicin in cultured primary human hepatocytes after infecting cells with adenovirus expressing scrambled siRNA (controls) or siHNF4-I

Primary human hepatocytes from four different donors were treated with control (scrambled siRNA) or siHNF4-I in the presence or absence of rifampicin in triplicate and analyzed for mRNA expression of CYP2C8, CYP2C9, CYP2C19, and HNF4α. Data represent the mean ± S.D.

Fold ChangesHNF4α2C82C92C19
Rif/control1.06 ± 0.11 (N.S.)6.57 ± 1.79b,c3.00 ± 0.70b,c1.84 ± 0.31b,c
siHNF4/Rif-siHNF41.31 ± 0.24a2.68 ± 1.40a1.53 ± 0.671.13 ± 0.21

  • Rif, rifampin; N.S., not significant.

  • ↵a Fold change significantly greater than 1.00, p < 0.05.

  • ↵b p < 0.01.

  • ↵c Fold induction for Rif/control significantly greater than fold induction for siHNF4/Rif versus siHNF4, p < 0.01 in all cases.