Primer sequences for target genes measured at the mRNA level by real-time quantitative RT-PCR
| Target | Forward and Reverse PCR Primer Sequences | PCR Product Size | Reference |
|---|---|---|---|
| base pair | |||
| AHR | 5′-GCTGTGATGCCAAAGGGCAGCT-3′ | 100 | New design |
| 5′-TGAAGCATGTCAGCGGCGTGGAT-3′ | |||
| CYP1A1 | 5′-GAATGCCAATGTCCAGCTCTCA-3′ | 115 | Tijet et al., 2006 |
| 5′-TACCAGGTACATGAGGCTCCAA-3′ | |||
| CYP1A2 | 5′-CGAGGGACACCTCACTGAAT-3′ | 93 | New design |
| 5′-TCCACTGCTTCTCATCATGGTT-3′ | |||
| CYP1B1 | 5′-CTCATCCTCTTTACCAGATACCCG-3′ | 117 | Vondrácek et al., 2006 |
| 5′-GACGTATGGTAAGTTGGGTTGGTC-3′ | |||
| PORa | 5′-GCCTGCCTGAGATCGACAAG-3′ | 64 | Muguruma et al., 2006 |
| 5′-GGGTCGCCCTCTCCGTATGT-3′ | |||
| β-Actin | 5′-GACCCAGATCATGTTTGAGACCTTC-3′ | 109 | Tijet et al., 2006 |
| 5′-GGAGTCCATCACAATGCCAGTG-3′ |
↵a The POR primer sequences were originally designed on the basis of the mouse cDNA sequence. The underlined and bolded base was changed to match the rat cDNA sequence.