P450 | Probe Substrate | Isoform-Specific Metabolite | Substrate Concentration | HLM Conc. | rhP450 Conc.a |
---|---|---|---|---|---|
μM | mg/ml | pmol/ml | |||
1A2 | Phenacetinb | O-Deethylation | 0.5 | 0.5 | 76 |
2C8 | Paclitaxel | 6α-Hydroxylation | 0.5 | 0.5 | 185 |
2C9 | Diclofenacb | 4-Hydroxylation | 5 | 0.25 | 89 |
S-Warfarin | 7-Hydroxylation | 0.5 | 0.5 | 179 | |
Tolbutamide | Hydroxylation | 1 | 0.5 | 179 | |
2C19 | S-Mephenytoin | 4-Hydroxylation | 1 | 1.0 | 213 |
2D6 | Bufuralolb | 1-Hydroxylation | 0.5 | 0.25 | 27 |
Dextromethorphan | O-Demethylation | 0.5 | 0.25 | 27 | |
3A4 | Midazolamb | 1′-Hydroxylation | 1 | 0.25 | 32 |
Testosterone | 6β-Hydroxylation | 1 | 0.25 | 32 | |
Nifedipine | Hydroxylation | 1 | 0.25 | 32 |