TABLE 4

ISEF determined based on metabolite formation and comparison with ISEF determined using substrate depletion

P450	Probe Substrate	HLM		rhP450		ISEF (Metabolite Formation)		ISEF (Substrate Depletion): ISEF-CL_int^{^c}
P450	Probe Substrate	V_max	K_m	V_max	K_m	VISEF^{^a}	CLISEF^{^b}	ISEF (Substrate Depletion): ISEF-CL_int^{^c}
		pmol per min/mg	μM	pmol per min/pmol P450	μM
1A2	Phenacetin	511	47	52	34	0.19	0.14	0.17
2C9	Diclofenac	1609	17.7	29	9.7	0.76	0.42	0.54
2D6	Bufuralol	135	11	52	8.3	0.32	0.23	0.15
3A4	Midazolam	973	2.94	21	2.89	0.33	0.32	0.21

↵a VISEF-calculated on the basis of V_max (eq. 1).
↵b CLISEF-calculated on the basis of CL_int = V_max/K_m (eq. 2).
↵c ISEF-CL_int calculated on the basis of measured substrate depletion, CL_int (eq. 3).