TABLE 3

Enzyme kinetic parameters of the formation of AZT glucuronide

Apparent Km and Vmax for the formation of metabolites when AZT was incubated with diabetic and nondiabetic HLMs and HKMs, respectively.

NondiabeticDiabeticP Value
HLM incubations with AZT
    AZT glucuronide
        Vmax [pmol/(min · pmol P450)]1214 ± 37274 ± 16P < 0.001
        Km (μM)330 ± 20307 ± 43NS
        Vmax/Km [pl/(min · pmol P450)]3.67 ± 0.300.87 ± 0.08P < 0.001
        Ksi (μM)200200
HKM incubations with AZT
    AZT glucuronide
        Vmax [pmol/(min · pmol P450)]20.5 ± 0.56.52 ± 0.45P < 0.001
        Km (μM)519 ± 49519 ± 31NS
        Vmax/Km [pl/(min · pmol P450)]0.040 ± 0.0040.013 ± 0.001P = 0.010
        Ksi (μM)150150

  • NS, nonsignificant at P > 0.05; plus-minus values are mean ± S.E.