Substrates | Column | Eluent (A/B) | Flow Rate | Gradient | Detection | Quantitation (Source) | Reference |
---|---|---|---|---|---|---|---|
ml/min | nm | ||||||
1-Naphthol | Hypersil BDS C18, 5 μm, 4 × 150 mm | 50 mM Phosphate buffer, pH 3/methanol | 1 | Isocratic, 60% A | Fluorescence, 285/335 | Authentic standard (Sigma-Aldrich) | Kurkela et al. (2003) |
Diclofenac | Acquity BEH C18 1.7 μm, 2.1 × 50 mm | 0.1% Formic acid/ACN | 0.5 | 80, 80, 5, 5, 80% A at 0, 1, 6, 6.1, 6.5 min | UV 283 | Parent compound absorbance | This work |
Epitestosterone and testosterone | Acquity BEH C18 1.7 μm, 1.0 × 100 mm | 0.1% Formic acid/ACN | 0.2 | 90, 45, 10, 90, 90% A at 0, 3, 3.5, 3.6, 6 min | UV 246 | Parent compound absorbance | Sten et al. (2008) |
Estradiol | Chromolith SpeedROD RP-18e 2 μm, 50 × 4.6 mm | 50 mM phosphate buffer, pH 3/methanol | 1, 1, 2, 2, 1 at 0, 10, 10.5, 23, 25 min | Isocratic, 60% A | Fluorescence, 216/316 | Authentic standard (Sigma-Aldrich) | This work |
Ethinylestradiol | Chromolith SpeedROD RP-18e 2 μm, 50 × 4.6 mm | 50 mM Phosphate buffer, pH 3/methanol | 1, 1, 3, 3, 1 at 0, 7, 7.5, 15, 17 min | Isocratic, 60% A | Fluorescence, 280/305 | Radiolabeled glucuronide; see Materials and Methods | Luukkanen et al. (2005) |
Indomethacin | Acquity BEH C18 1.7 μm, 2.1 × 50 mm | 0.1% Formic acid/ACN | 0.5 | 80, 80, 5, 5, 80% A at 0, 1, 6, 6.1, 6.5 min | UV 320 | Parent compound absorbance | This work |
Oxazepam | Acquity BEH C18 1.7 μm, 2.1 × 50 mm | 10 mM Ammonium acetate, pH 8/ACN | 0.5 | 95, 50, 5, 95, 95% A at 0, 4, 5, 6, 6.5 min | UV 230 | Parent compound absorbance | This work |
Propofol | Acquity BEH C18 1.7 μm, 2.1 × 50 mm | 0.1% Formic acid/ACN | 0.5 | 95, 50, 5, 95, 95% A at 0, 4, 5, 6, 6.5 min | UV 270 | Parent compound absorbance | This work |
R- and S-propranolol | Hypersil BDS C18, 5 μm, 4 × 150 mm | 50 mM Phosphate buffer, pH 3/methanol | 1 | Isocratic, 60% A | Fluorescence, 230/342 | Radiolabeled glucuronide; see Materials and Methods | This work |
TFP | Acquity BEH C18 1.7 μm, 2.1 × 50 mm | 0.1% Formic acid/ACN | 0.5 | 95, 95, 10, 10, 95% A at 0, 1, 6, 6.1, 6.5 min | UV 258 | Parent compound absorbance | This work |
AZT | Acquity BEH C18 1.7 μm, 2.1 × 50 mm | 0.1% Formic acid/ACN | 0.5 | 95, 95, 70, 10, 95% A at 0, 1, 6, 6.1, 6.5 min | UV 267 | Parent compound absorbance | This work |
ACN, acetonitrile.