Derived kinetic parameters for acetaminophen glucuronidation by recombinant human UGT enzymes
Data are presented as parameter ± S.E. of parameter fit.
| Enzyme | Equation | Km or S50 | Vmax | Ksi | n |
|---|---|---|---|---|---|
| mM | pmol/min · mg | nM | |||
| UGT1A1 | Hill | 8.7 ± 0.3 | 26 ± 0.4 | N.A. | 1.38 ± 0.04 |
| UGT1A6 | MM | 2.2 ± 0.05 | 367 ± 2.6 | N.A. | N.A. |
| UGT1A9 | Hill | 25 ± 0.4 | 1087 ± 12 | N.A. | 1.60 ± 0.02 |
| UGT2B15 | SI | 7.6 ± 1.0 | 344 ± 15 | 36 ± 5.0 | N.A. |
MM, Michaelis-Menten; SI, substrate inhibition, N.A., not applicable.