Effect of niflumic acid (2.5, 50, and 100 μM) on the kinetics of APAP glucuronidation by HLM

Kinetic parameters were derived from fitting with the Michaelis-Menten equation. Data are presented as parameter ± S.E. of parameter fit.

Liver No.Control (no NFA)2.5 μM NFA50 μM NFA100 μM NFA
KmVmaxKmVmaxKmVmaxKmVmax
mMpmol/min · mgmMpmol/min · mgmMpmol/min · mgmMpmol/min · mg
HL1218 ± 0.22355 ± 1013 ± 0.11872 ± 714 ± 0.31702 ± 1714 ± 0.61592 ± 31
HL1322 ± 0.12423 ± 6517 ± 0.91868 ± 5015 ± 0.11290 ± 415 ± 0.11180 ± 3
HL4011 ± 0.0093376 ± 1.09.9 ± 0.022792 ± 1.89.9 ± 0.092290 ± 98.8 ± 0.742111 ± 60