TABLE 8

Comparison of incubation conditions along with IC₅₀ values (and the fold shift) with and without NADPH (curves B and C) for the dilution methods

All IC₅₀ values are based on the final or postdilution concentrations.

P450 Enzyme	Substrate/Inhibitor	This Study						Perloff et al., 2009 ^{^a}						Obach et al., 2007 ^{^a}
		[HLM]	Time	Fold Dilution	IC₅₀		Fold Shift (B/C)	[HLM]	Time	Fold Dilution	IC₅₀		Fold Shift (B/C)^{^a}	[HLM]	Time	Fold Dilution	IC₅₀		Fold Shift (B/C)^{^a}
		[HLM]	Time	Fold Dilution	B	C	Fold Shift (B/C)	[HLM]	Time	Fold Dilution	B	C	Fold Shift (B/C)^{^a}	[HLM]	Time	Fold Dilution	B	C	Fold Shift (B/C)^{^a}
		mg · ml⁻¹	min		μM			mg · ml⁻¹	min		μM			mg · ml⁻¹	min		μM
CYP1A2	Phenacetin/furafylline	0.1	5	10	3.4	0.036	94	0.2	10	10	8.4	0.021	400	0.03	30	10	1.5	0.027	56
CYP2B6	Bupropion/ticlopidine	0.1	5	10	0.20	0.039	5.1	0.1	5	10	0.84	0.048	18	0.05	20	10	0.13	0.031	4.2
CYP2C8	Amodiaquine/gemfibrozil glucuronide	0.0125	5	10	27	0.28	96	0.02	5	10	26	0.46	57	0.025	10	10	N.D.	N.D.	N.D.
CYP2C9	Diclofenac/tienilic acid	0.1	5	10	0.92	0.057	16	0.05	5	10	1.7	0.049	35	0.03	10	10	0.43	0.027	16
CYP2C19	S-Mephenytoin/S-fluoxetine	0.1	5	10	61	1.4	44	0.3	10	5	85	3.1	27	0.2	40	10	N.D.	N.D.	N.D.
CYP2D6	Dextromethorphan/Paroxetine	0.1	5	10	1.5	0.053	28	0.1	5	10	1.1	0.066	17	0.03	10	10	0.23	0.012	19
CYP3A4	Midazolam/azamulin	0.05	5	10	0.34	0.031	11	0.02	5	10	0.15	0.0025	60	0.03	4	10	N.D.	N.D.	N.D.
	Midazolam/diltiazem	0.05	5	10	78	1.2	65	0.02	5	10	100	3.4	29	0.03	4	10	54	3.7	15
	Midazolam/verapamil	0.05	5	10	25	0.41	61	0.02	5	10	25	0.34	73	0.03	4	10	12	0.12	100
CYP3A4	Testosterone/azamulin	0.1	5	10	0.46	0.044	10	0.05	10	10	0.098	0.0077	13	0.03	10	10	N.D.	N.D.	N.D.
	Testosterone/diltiazem	0.1	5	10	138	2.2	63	0.05	10	10	130	2.8	46	0.03	10	10	55	1.8	31
	Testosterone/verapamil	0.1	5	10	29	0.38	76	0.05	10	10	28	0.27	104	0.03	10	10	8.2	0.15	55