TABLE 3

TDI kinetic parameters generated in human hepatocytes and human liver microsomes

Parameters	Human Hepatocyte: Add Method		Human Hepatocyte: Dilution Method		Human Hepatocyte: Add Method (Mean)	Human Hepatocyte: Dilution Method (Mean)	HLM^{^a} (Mean ± S.D.)
Parameters	Assay 1	Assay 2	Assay 1	Assay 2	Human Hepatocyte: Add Method (Mean)	Human Hepatocyte: Dilution Method (Mean)	HLM^{^a} (Mean ± S.D.)
Diltiazem
K_I	6.6	6.0	10.5	7.4	6.3	8.9	1.53 ± 0.565
k_inact	0.0162	0.0219	0.0234	0.0222	0.019	0.0228	0.024 ± 0.011
k_inact/K_I	0.0024	0.0037	0.0022	0.003	0.0031	0.0026	0.016 ± 0.001
Erythromycin
K_I	61.3	58.7	67.1	68.6	60.0	67.9	5.33 ± 2.57
k_inact	0.0856	0.0760	0.0868	0.0712	0.081	0.079	0.061 ± 0.002
k_inact/K_I	0.0014	0.0013	0.0013	0.0010	0.0013	0.0012	0.013 ± 0.005
Verapamil
K_I	2.4	1.4	2.2	2.1	1.9	2.1	3.19 ± 0.251
k_inact	0.02	0.0203	0.0249	0.0295	0.02	0.0272	0.076 ± 0.004
k_inact/K_I	0.0083	0.0149	0.0113	0.0141	0.0116	0.0127	0.024 ± 0.003
TAO
K_I	2.7	3.3	3.7	3.2	3.0	3.4	0.440 ± 0.140
k_inact	0.237	0.238	0.356	0.382	0.238	0.369	0.078 ± 0.053
k_inact/K_I	0.088	0.072	0.098	0.119	0.0798	0.1081	0.205 ± 0.180