TABLE 1

Difference in kinetic parameters of CPA 4-hydroxylation among CYP2B6 variants expressed in Sf9 cells

Data are represented by means ± S.E.

Kmkcatkcat/Km
mMnmol per min/nmol P450ml per min/nmol P450
CYP2B6.12.68 ± 0.26301 ± 10.3112 ± 11.5 (100%)
CYP2B6.42.75 ± 0.55223 ± 16.2*81.3 ± 17.3 (72.6%)
CYP2B6.61.62 ± 0.18297 ± 10.4†184 ± 21.2† (164%)

  • ↵* P < 0.05 versus CYP2B6.1 (Tukey-Kramer multiple comparison test).

  • ↵† P < 0.05 versus CYP2B6.4 (Tukey-Kramer multiple comparison test).

  • The variant to wild-type ratios are indicated in parentheses.