TABLE 2

Difference in kinetic parameters of EFV 8-hydroxylation among CYP2B6 variants expressed in Sf9 cells

Data are represented by mean ± S.E.

Kmkcatkcat/Km
μMnmol per min/nmol P450μl per min/nmol P450
CYP2B6.17.70 ± 1.559.06 ± 0.471.18 ± 0.25 (100%)
CYP2B6.49.16 ± 1.6215.3 ± 0.74***1.67 ± 0.31 (142%)
CYP2B6.612.4 ± 1.517.33 ± 0.27†††0.59 ± 0.08 (50.0%)
  • *** P < 0.001 versus CYP2B6.1 (Tukey-Kramer multiple comparison test).

  • P < 0.05 versus CYP2B6.4 (Tukey-Kramer multiple comparison test).

  • ††† P < 0.001 versus CYP2B6.4 (Tukey-Kramer multiple comparison test).

  • The variant to wild-type ratios are indicated in parentheses.