TABLE 2

Cytochrome P450 proteins identified from tryptic digests of protein bands 7, 8, and 9 resolved using SDS-PAGE

Identification was carried out by submitting MALDI-TOF MS data to a peptide mass fingerprint search using Mascot and suitable databases. The P450 region was shown to be 45 to 60 kDa.

Solubilizing AgentSDSTriton X-100Urea + Thiourea
Band 7CYP2E1CYP2E1
Band 8CYP2D25CYP2D25CYP2D25
CYP2DCYP2DCYP2D
CYP3ACYP3ACYP3A
CYP2A19
Band 9CYP2A19CYP2A19