HEK293-MDR1 Vesicles | Caco-2 Cell Monolayers | ||||
---|---|---|---|---|---|
Batch | [3H]NMQ Uptake | P-gp Content | Culturing Time | [3H]Digoxin Efflux Ratio | P-gp Content |
pmol · min−1 · mg protein−1 | pmol P-gp/mg total protein | pmol P-gp/mg total protein | |||
Batch 3 | 61 ± 11 | 8.15 | 10 Days | 4.6 ± 0.1 | 4.00 |
Batch 6 | 220 ± 30 | 32.0 | 29 Days | 10.2 ± 2.4 | 7.89 |
Fold difference (batch 6/batch 3) | 3.6 | 3.9 | Fold difference (29 days/10 days) | 2.2 | 2.0 |